NOT KNOWN FACTS ABOUT 2-FDCK HCL

The lab states fixation will cause the dyes to leak in the cells. I feel the assumption is that fixation could damage cell membranes. I did look for a paper which describes fixation utilizing methanol or acetic acid, immediately after staining having a DCFDA by-product. DCFDA is the reagent that stains ROS during the kit ab113851, and I suspect tha